Macrogen Korea 10F, 254 Beotkkot-ro Geumcheon-gu, Seoul 08511, Rep. of Korea Tel : +82-2-2180-7000 [email protected] Macrogen Singapore Synapse #05-18, Primer Sequence M13 Forward (-20) 5'{GTA AAA CGA CGG CCA G}3' M13 Reverse (-20) 5'{CAG GAA ACA GCT ATG AC}3' SP6 5'{ATT TAG GTG ACA CTA TAG}3' T3 5'{ATT AAC CCT CAC TAA AGG GA}3' T7 Promoter 5'{TAA TAC GAC TCA CTA TAG GG}3' T7 Terminator 5'{GCT AGT TAT TGC TCA GCG G}3' pcDNA3.1/BGH Reverse 5'{TAG AAG GCA CAG TCG AGG}3' 5'-pGEX 5'{GGG CTG GCA … Plasmid pCMV_ABEmax from Dr. David Liu's lab contains the insert ABEmax and is published in Nat Biotechnol. GAPDH served as a loading control. - MALDI-TOF QC - Confirms purification by HPLC - 70 types of primers for sequencing, 17 types of primers for microbe identification and 5 types of random primers Refer 3–5 for the sequence and location of the priming sites. Primer Name Catalog # pmoles BGH Reverse N575-02 358 TAGAAGGCACAGTCGAGG CMV Forward N622-02 306 Primers on the Standard Primer List (below) are provided free of charge. Continued on next page . Features - 5nmol of ≥ 95% pure primer (PAGE purification). For 96-well format, provide at least 120 µl of primer for each plate. 5′ end of ampicillin resistance gene, reverse primer: AUG1 Forward: CAATTTACATCTTTATTTATTAACG For Pichia vectors with AUG1 promoter, forward primer: AUG1 Reverse: GAAGAGAAAAACATTAGTTGGC For Pichia vectors with AUG1 promoter, reverse primer: BGH Reverse: TAGAAGGCACAGTCGAGG Bovine growth hormone terminator, reverse primer: … For more information, refer to www.lifetechnologies.com or contact Technical Support (see page 12). Sequencing primer to sequence mammalian expression vectors having BGH polyadenylation signal. Use this program to produce a useful reference figure, particularly when you have designed a large number of primers for a particular template. Invitrogen™ BGH Reverse Primer . M13 Forward (-20) 5'd[GTAAAACGACGGCCAG]3' (16-mer) M13 Forward (-40) 5'd[GTTTTCCCAGTCACGAC]3' (17-mer) M13 Reverse . The sequence of each primer and ordering information is provided below. (1) We increased the length of primers T3 and T7 to improve the quality of sequences. Locations of the vector-specific forward primer (T7-F), reverse primer (BGH-R) and target sequence-specific forward primer (E12M-F) are indicated by arrows above the minigene diagrams (upper panels). Sequence: Length: Tm [°C] GC [%] bgh reverse primer: gctgg caact agaag gcaca g: pcold-f1 primer *7: gtaag gcaag tccct tcaag ag: pcold-r primer *7: cgcat tctca ttgca cccaa: pcoldtf-f1 primer *7: ccact ttcaa cgagc tgatg: rv-p: ggaaa cagct atgac catga ttac: m13-20: cgacg ttgta aaacg acggc cagt: m13-47b *8: ggcga aaggg ggatg tgctg caag: 10f *9: gtttg atcct ggctc a: Primer sequences can be found here: M13 Forward GTA AAA CGA CGG CCA GTG M13 Reverse GGA AAC AGC TAT GAC CAT G T7 Promo Add to cart Includes: Primer is supplied as 2µg which equals 358 pMoles. Primer Sequence For your convenience, we offer a custom primer synthesis service. Primer Map Restriction endonuclease cut sites, and the protein translations of the DNA sequence can also be shown. CMV Forward 5´-CGCAAATGGGCGGTAGGCGTG-3´ N622-02 BGH Reverse 5´-TAGAAGGCACAGTCGAGG-3´ N575-02 General Molecular 1. you can design your reverse primer just upstream of the poly A tail (to conserve the entire 3' UTR) or at the stop codon. 20 μL *TE buffer, pH 8.0: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 . $377.00 / Each; Qty. S.No Primer Name Primer Sequence 1 M13 Reverse (-27) 5′-GGA AAC AGC TAT GAC CAT G-3′ 2 M13 Forward (-41) 5′-GGT TTT CCC […] M13 forward sequencing primer (-40): GTTTTCCCAGTCACGAC M13 forward sequencing primer (-47): CGCCAGGGTTTTCCCAGTCACGAC M13 reverse sequencing primer: (-24): AACAGCTATGACCATG Macrogen Europe B.V. Meibergdreef 31 1105 AZ, Amsterdam, the Netherlands Tel: +31 20 333 7563 Email: [email protected] Macrogen Korea 10F, 254 Beotkkot-ro TAGAAGGCACAGTCGAGG. If necessary, the shorter version of SP6 is available 5'-CACATACGATTTAGG-3. Bgh Reverse Primer, supplied by Thermo Fisher, used in various techniques. Primer Sequence CMV forward . Customer Provided Primers. DuetDOWN1: GATTATGCGGCCGTGTACAA: For pETDuet, pACYCDuet vectors (7) These primers work in the Duet vectors for co-expression of proteins. Primer Sequence Amount T7 5´-TAATACGACTCACTATAGGG-3´ 328 pmoles GFP Reverse 5´-GGGTAAGCTTTCCGTATGTAGC-3´ 296 … GENEWIZ offers a variety of free universal primers for sequencing. Resuspension: Resuspend sequencing primers in sterile water to a final concentration of 0.1 µg/µl. It binds to a wide variety of DNA templates. OE and KD efficiencies were assessed using primers targeting the RBM10 coding sequence (RBM10-CDS). Standard Primers. Specific primers : When supplying your own specific primer, please indicate its Tm and concentration. Primer Sequence Catalog no. Bioz Stars score: 89/100, based on 73 PubMed citations. Identity is confirmed by mass spectrometry* and purity is … BGH Reverse primers to confirm that your gene is in the correct orientation for expression and contains an ATG and a stop codon. Sequences. Bioz Stars score: 95/100, based on 37 PubMed citations. Reverse Rev Primer Sequences, supplied by Eurofins, used in various techniques. 2 µg/μL in TE buffer, pH 8.0 . Universal primers are complementary to nucleotide sequences used for the amplification of a very similar gene that related to a specific Genus. Refer to the diagrams on pages 3–5 for the sequence and location of the primer binding sites. suggest using the T7 Promoter and BGH Reverse primer sequences. ReadyMade Primers are stocked oligonucleotides for sample preparation, PCR, sequencing, and gene expression analysis of common genes. Primer Sequence to the diagrams on pages We recommend that you sequence your construct with the T7 Promoter and BGH Reverse primers (see page 12 for ordering information) to confirm that your gene is fused in frame with the V5 epitope and the C-terminal polyhistidine tag. Two micrograms of each primer are supplied. Users in our new CLIMS Online Ordering and Data Management System have access to the Updated GENEWIZ Universal Primer list (see below). ZERO BIAS - scores, article reviews, protocol conditions and more Store resuspended primers at –20°C. N57502. For sequencing from the 3' end of mammalian expression vectors containing the BGH polyadenylation signal. Primer Sequence pMoles Supplied T7 Promoter 5´-TAATACGACTCACTATAGGG-3´ 328 BGH Reverse 5´-TAGAAGGCACAGTCGAGG-3´ 358 Genotype of TOP10 Cells 3 . (BGH Reverse Sequencing Primer) 0.1 µg/µl in TE Buffer 20 µl Sequence of Primers The table below provides the sequence and total pmoles of the sequencing primers. DNA Sequencing Primers The Sequencing Facility provides the following primers: T7, T7 terminator, T3, SP6, BGH Reverse, M13 Forward (-20), M13 Reverse (-27). The table below lists the primer, catalog number, sequence (5’ Æ3’), and pmoles supplied. 5'd[CAGGAAACAGCTATGAC]3' (17-mer) Primers should be provided in nuclease free water. 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