For 96-well format, provide at least 120 µl of primer for each plate. Manufacturer: Invitrogen™ N57502 Catalog No. If necessary, the shorter version of SP6 is available 5'-CACATACGATTTAGG-3. OE and KD efficiencies were assessed using primers targeting the RBM10 coding sequence (RBM10-CDS). Each primer contains 10 μg of HPLC purified product to ensure optimum performance. DuetDOWN1 gives a reverse read of T7 transcription start-1 MCS. TAGAAGGCACAGTCGAGG. Bioz Stars score: 95/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more The sequence of each primer and ordering information is provided below. Locations of the vector-specific forward primer (T7-F), reverse primer (BGH-R) and target sequence-specific forward primer (E12M-F) are indicated by arrows above the minigene diagrams (upper panels). As the largest gene synthesis provider in the USA with proven capability and reliability, GenScript now expands DNA sequencing services in North America to offer … 20 μL *TE buffer, pH 8.0: 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 . 5'd[CAGGAAACAGCTATGAC]3' (17-mer) Two micrograms of each primer are supplied. Invitrogen™ BGH Reverse Primer . Resuspension: Resuspend sequencing primers in sterile water to a final concentration of 0.1 µg/µl. S.No Primer Name Primer Sequence 1 M13 Reverse (-27) 5′-GGA AAC AGC TAT GAC CAT G-3′ 2 M13 Forward (-41) 5′-GGT TTT CCC […] For more information, refer to www.lifetechnologies.com or contact Technical Support (see page 12). Store resuspended primers at –20°C. - MALDI-TOF QC - Confirms purification by HPLC - 70 types of primers for sequencing, 17 types of primers for microbe identification and 5 types of random primers CMV-F. CGCAAATGGGCGGTAGGCGTG. Use this program to produce a useful reference figure, particularly when you have designed a large number of primers for a particular template. M13 forward sequencing primer (-40): GTTTTCCCAGTCACGAC M13 forward sequencing primer (-47): CGCCAGGGTTTTCCCAGTCACGAC M13 reverse sequencing primer: (-24): AACAGCTATGACCATG Primers should be provided in nuclease free water. Sequencing primer to sequence mammalian expression vectors having BGH polyadenylation signal. primers sequences; 3’ ad: 5’ (aga tgg tgc acg atg cac ag) 3’ 3 aox1: 5′ (gca aat ggc att ctg aca tcc) 3’ 5’ ad: 5’ (ttc gat gat gaa gat acc cc) 3’ 5 aox1: 5′ (gac tgg ttc caa ttg aca agc) 3’ bgh reverse: 5′ (tag aag gca cag tcg agg) 3′ bk reverse: 5′ (aca gga aac agc tat … Standard Primers. This plasmid is available through Addgene. (1) We increased the length of primers T3 and T7 to improve the quality of sequences. Customer Provided Primers. Sequences. For your convenience, we offer a custom primer synthesis service. N57502. Primer sequences can be found here: M13 Forward GTA AAA CGA CGG CCA GTG M13 Reverse GGA AAC AGC TAT GAC CAT G T7 Promo 3 . Plasmid Preparation 2 µg/μL in TE buffer, pH 8.0 . suggest using the T7 Promoter and BGH Reverse primer sequences. Primers . CMV Forward 5´-CGCAAATGGGCGGTAGGCGTG-3´ N622-02 BGH Reverse 5´-TAGAAGGCACAGTCGAGG-3´ N575-02 General Molecular Specific primers : When supplying your own specific primer, please indicate its Tm and concentration. The pcDNA ™ 3.4-TOPO ® TA Vector Kit contains the following primers to sequence your insert. Primers should be provided at a concentration of 10µM (picomoles/µl). GENEWIZ offers a variety of free universal primers for sequencing. Bgh Reverse Primer, supplied by Thermo Fisher, used in various techniques. BGH-Reverse. Primer Name Catalog # pmoles BGH Reverse N575-02 358 TAGAAGGCACAGTCGAGG CMV Forward N622-02 306 EGFP-C Primers The table below provides the sequence and pmoles of the T7 Promoter primer and the BGH Reverse primer. The subsequent recombinant PCR using CMV forward primer, located upstream of the cDNA sequence, and BGH reverse primer, located downstream of the cDNA sequence has been performed to fuse the overlapping mutant fragments. Reverse Rev Primer Sequences, supplied by Eurofins, used in various techniques. 5′ end of ampicillin resistance gene, reverse primer: AUG1 Forward: CAATTTACATCTTTATTTATTAACG For Pichia vectors with AUG1 promoter, forward primer: AUG1 Reverse: GAAGAGAAAAACATTAGTTGGC For Pichia vectors with AUG1 promoter, reverse primer: BGH Reverse: TAGAAGGCACAGTCGAGG Bovine growth hormone terminator, reverse primer: … Primer Sequence to the diagrams on pages We recommend that you sequence your construct with the T7 Promoter and BGH Reverse primers (see page 12 for ordering information) to confirm that your gene is fused in frame with the V5 epitope and the C-terminal polyhistidine tag. doi: 10.1038/nbt.4172. It must be provided in a separate tube at 10 uM. Primers. Sequence: Length: Tm [°C] GC [%] For sequencing from the 3' end of mammalian expression vectors containing the BGH polyadenylation signal. Primers on the Standard Primer List (below) are provided free of charge. Macrogen Europe B.V. Meibergdreef 31 1105 AZ, Amsterdam, the Netherlands Tel: +31 20 333 7563 Email: [email protected] Macrogen Korea 10F, 254 Beotkkot-ro CMV promoter, forward primer. BGH (bovine growth hormone) terminator, reverse primer. GAPDH served as a loading control. Plasmid pCMV_ABEmax from Dr. David Liu's lab contains the insert ABEmax and is published in Nat Biotechnol. It binds to a wide variety of DNA templates. Users in our new CLIMS Online Ordering and Data Management System have access to the Updated GENEWIZ Universal Primer list (see below). These free universal primers are being updated to reflect the needs of our customers. Refer to the diagrams on pages 3–5 for the sequences and locations of the priming sites. Refer 3–5 for the sequence and location of the priming sites. (2) Formerly named New-SP6. Primer Sequence CMV forward . Primer Sequence Catalog no. Kit Contents and Storage, continued . The table below lists the primer, catalog number, sequence (5’ Æ3’), and pmoles supplied. $377.00 / Each; Qty. Primer Map Restriction endonuclease cut sites, and the protein translations of the DNA sequence can also be shown. Primer Sequence pMoles Supplied T7 Promoter 5´-TAATACGACTCACTATAGGG-3´ 328 BGH Reverse 5´-TAGAAGGCACAGTCGAGG-3´ 358 Genotype of TOP10 Cells ™3.4 reverse sequencing primer . Refer to the diagrams on pages 3–5 for the sequence and location of the primer binding sites. Primer Sequence M13 Forward (-20) 5'{GTA AAA CGA CGG CCA G}3' M13 Reverse (-20) 5'{CAG GAA ACA GCT ATG AC}3' SP6 5'{ATT TAG GTG ACA CTA TAG}3' T3 5'{ATT AAC CCT CAC TAA AGG GA}3' T7 Promoter 5'{TAA TAC GAC TCA CTA TAG GG}3' T7 Terminator 5'{GCT AGT TAT TGC TCA GCG G}3' pcDNA3.1/BGH Reverse 5'{TAG AAG GCA CAG TCG AGG}3' 5'-pGEX 5'{GGG CTG GCA … M13 Forward (-20) 5'd[GTAAAACGACGGCCAG]3' (16-mer) M13 Forward (-40) 5'd[GTTTTCCCAGTCACGAC]3' (17-mer) M13 Reverse . ReadyMade Primers are stocked oligonucleotides for sample preparation, PCR, sequencing, and gene expression analysis of common genes. BGH Reverse primers to confirm that your gene is in the correct orientation for expression and contains an ATG and a stop codon. Continued on next page . (BGH Reverse Sequencing Primer) 0.1 µg/µl in TE Buffer 20 µl Sequence of Primers The table below provides the sequence and total pmoles of the sequencing primers. We recommend that you sequence your construct with the T7 Forward and BGH Reverse primers (see page 12 for ordering information) to confirm that your gene is fused in frame with the N-terminal His tag and the enterokinase site. 1. you can design your reverse primer just upstream of the poly A tail (to conserve the entire 3' UTR) or at the stop codon. Bioz Stars score: 89/100, based on 73 PubMed citations. T7 Primer 5' TAA TAC GAC TCA CTA TAG GG 3' promoter T7 Rev Primer 5' GCT AGT TAT TGC TCA GCG G 3' terminator SP6 Primer 5' TAT TTA GGT GAC ACT ATA G 3' promoter T3 Primer 5' ATT AAC CCT CAC TAA AGG GA 3' promoter CMV Forward 5' CGC AAA TGG GCG GTA GGC GTG 3' BGH Reverse Primer 5' TAG AAG GCA CAG TCG AGG 3' Primer Sequence Amount T7 5´-TAATACGACTCACTATAGGG-3´ 328 pmoles GFP Reverse 5´-GGGTAAGCTTTCCGTATGTAGC-3´ 296 … Macrogen Korea 10F, 254 Beotkkot-ro Geumcheon-gu, Seoul 08511, Rep. of Korea Tel : +82-2-2180-7000 [email protected] Macrogen Singapore Synapse #05-18, Features - 5nmol of ≥ 95% pure primer (PAGE purification). Add to cart Includes: Primer is supplied as 2µg which equals 358 pMoles. bgh reverse primer: gctgg caact agaag gcaca g: pcold-f1 primer *7: gtaag gcaag tccct tcaag ag: pcold-r primer *7: cgcat tctca ttgca cccaa: pcoldtf-f1 primer *7: ccact ttcaa cgagc tgatg: rv-p: ggaaa cagct atgac catga ttac: m13-20: cgacg ttgta aaacg acggc cagt: m13-47b *8: ggcga aaggg ggatg tgctg caag: 10f *9: gtttg atcct ggctc a: DuetDOWN1: GATTATGCGGCCGTGTACAA: For pETDuet, pACYCDuet vectors (7) These primers work in the Duet vectors for co-expression of proteins. Primer Sequence Standard Primer @ GATC 1 31.01.2019 Standard Primer GATC. Universal primers are complementary to nucleotide sequences used for the amplification of a very similar gene that related to a specific Genus. 2018 May 29. pii: nbt.4172. 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